Literature summary extracted from

Stone, L.A.; Jackson, G.S.; Collinge, J.; Wadsworth, J.D.; Clarke, A.R.
Inhibition of proteinase K activity by copper(II) ions (2007), Biochemistry, 46, 245-252.

Activating Compound

EC Number	Activating Compound	Comment	Organism	Structure
3.4.21.64	additional information	presence of calcium ions, up to 10 mM, has no effect on enzymatic activity	Tritirachium album Limber

Application

EC Number	Application	Comment	Organism
3.4.21.64	medicine	PK activity in the presence of brain correlates precisely with the concentration of Cu2+ ions that prevent PK digestion of cellular prion protein and other brain proteins. Apparent resistance of cellular prion protein to proteolysis by PK appears to be directly attributable to the inhibition of PK activity by copper-(II) ions	Tritirachium album Limber

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
3.4.21.64	Cu2+	at equilibrium two to three copper ions bind stoichiometrically to PK and destroy its activity. Initial reversible and weak binding phase and a slower, irreversible abolition of activity with a half-time of 6 min at saturating copper ion concentrations. PK digestion of cellular prion proteins and other proteins in brain homogenate is inhibited in a concentration-dependent manner at concentrations of more than 1 mM. Presence of calcium ions, up to 10 mM, has no effect on copper inhibition	Tritirachium album Limber
3.4.21.64	additional information	not inhibited by Zn2+ or Mn2+	Tritirachium album Limber

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.4.21.64	Tritirachium album Limber	-	-	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.4.21.64	additional information	PK is very effective in destroying cellular prion proteins and endogenous proteases present in brain homogenate	Tritirachium album Limber	?	-	?
3.4.21.64	p-nitrophenyl acetate + H2O	-	Tritirachium album Limber	p-nitrophenol + acetate	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
3.4.21.64	Proteinase K	-	Tritirachium album Limber

pH Range

EC Number	pH Minimum	pH Maximum	Comment	Organism
3.4.21.64	6.7	7.4	-	Tritirachium album Limber

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Release 2023.1 (January 2023)